| Autor: |
Zheng Guo, Dong-Yan Chen, Jing Du, Shu-Kai Li, Qiu-Ping Zhang, Atsuko Shimada, Li Gao, Yi-Yao Liu, Hiroyuki Takeda, Jiang-Fan Yin, Xi-Qiao Feng, Toru Kawanishi, Li-Sha Zheng, Liu-Yuan Guan, Yubo Fan |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.1101/2021.02.25.432863 |
| Popis: |
The left-right symmetry breaking of vertebrate embryos requires fluid flow (called nodal flow in zebrafish). However, the molecular mechanisms that mediate the asymmetric gene expression regulation under nodal flow remain elusive. In this paper, we report that heat shock factor 1 (HSF1) is asymmetrically activated in the Kuppfer’s vesicle at the early stage of zebrafish embryos in the presence of nodal flow. Deficiency in HSF1 expression caused a significantsitus inversusand disrupted gene expression asymmetry of nodal signaling proteins in zebrafish embryos. Further studies demonstrated that HSF1 could be immediately activated by fluid shear stress. The mechanical sensation ability of HSF1 is conserved in a variety of mechanical stimuli in different cell types. Moreover, cilia and the Ca2+-Akt signaling axis are essential for the activation of HSF1 under mechanical stressin vitroandin vivo. Considering the conserved expression of HSF1 in organisms, these findings unveil a fundamental mechanism of gene expression regulation triggered by mechanical clues during embryonic development and other physiological and pathological transformations. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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