| Autor: |
Thomas M. Jovin, Charles K. Singleton, L H Hanau, Robert D. Wells, M W Kilpatrick, J Kłysik, David A. Zarling, B F Erlanger |
| Rok vydání: |
1984 |
| Předmět: |
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| Zdroj: |
Journal of Biological Chemistry. 259:7268-7274 |
| ISSN: |
0021-9258 |
| DOI: |
10.1016/s0021-9258(17)39867-8 |
| Popis: |
The large intervening sequences ( IVS2 ) of three human fetal globin genes contain tracts of alternating purine-pyrimidine sequences approximately 40-60 base pairs in length which adopt left-handed Z DNA helices under the influence of negative supercoiling. The amount of negative supercoiling (approximately 0.045) required for the right- to left-handed transitions is within the physiological range. The structural aberrations between the right- and left-handed helices were mapped by sequencing the S1 nuclease cleavage sites. Two-dimensional gel electrophoretic analyses of the supercoil-induced relaxation served to characterize the type and length of left-handed structure. Furthermore, binding studies with several types of antibodies confirmed the presence of left-handed helices. Since these simple sequences appear to be hotspots for recombination and gene conversion, unusual DNA conformations may participate in genetic expression. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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