| Popis: |
Background: Although fibronectin (FN) is an important extracellular glycoprotein involved in periodontal wound healing, it is not clear whether the application of exogenous fibronectin (ExoFN) offers any clinical benefit. The purpose of this preliminary in vitro study was to determine the binding of FN from three different sources, viz. endogenous EDTA-plasma, endogenous serum and exogenous commercial purified human fibronectin in PBS buffer, to demineralized and non-demineralized root powder. Method: The binding of FN to a known quantity of mineralized and non-demineralized root powder by overnight incubation at 15°C was studied by enzyme immunoassay (EIA) technique. The criteria for optimal performance of EIA procedure for the determination of FN was established. Particle size of powdered root structure was standardized using a Vibratory Sieve Shaker. Results: The EDTA-plasma and the serum FN exhibited binding of (17.8 ± 2.1 µg) and (6.5 ± 4.5 µg), respectively, to the non-demineralized root powder. However, the binding was only significant for the EDTA-plasma FN (p |
Plný text