Long form PRLR (lPRLR) regulates genes involved in the triacylglycerol synthesis in goat mammary gland epithelial cells
| Autor: | Yongqing Yi, Jun Luo, Hui Wang, Tianying Zhang, Huaiping Shi |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
ACACA Peroxisome proliferator-activated receptor gamma Prolactin receptor 0402 animal and dairy science 04 agricultural and veterinary sciences Biology 040201 dairy & animal science Molecular biology Sterol regulatory element-binding protein 03 medical and health sciences chemistry.chemical_compound Transmembrane domain 030104 developmental biology Food Animals chemistry Biochemistry Animal Science and Zoology Protein kinase B Peptide sequence Fatty acid synthesis |
| Zdroj: | Small Ruminant Research. 139:7-14 |
| ISSN: | 0921-4488 |
| Popis: | The prolactin receptor (PRLR) is a single-pass transmembrane receptor belonging to the class-1 cytokine receptor superfamily and known to play a crucial role in lactation and milk synthesis. However, very little information is available on the roles of PRLR in the regulation of lipid metabolism in the goat mammary gland. In this study, we cloned the whole cDNA of goat long form PRLR (lPRLR). The full-length cDNA of lPRLR was comprised of a 327-bp 5′-untranslated region, 1746-bp open reading frame and 183-bp 3′-UTR. The deduced amino acid sequence encoded 581 amino acids (aa) with signal peptide (24 aa) and single transmembrane domain (22 aa). Homology alignments revealed that the goat lPRLR was highly conserved among sheep, bovine, and buffalo. Real-time quantitative PCR suggested that goat lPRLR was predominantly expressed in the muscle and liver. Treatment of goat mammary epithelial cells (GMECs) with prolactin resulted in Protein Kinase B (AKT) phosphorylation, elevated Peroxisome Proliferator-Activated Receptor γ (PPARG), Sterol Regulatory Element-Binding Protein-1 (SREBP1), Fatty Acid Synthesis (FASN), and Acetyl-Coenzyme A Carboxylase Alpha (ACACA) mRNA levels, and significantly increased the triglyceride content (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect