Acoustic Wave Sparsely-Activated Localization Microscopy (AWSALM): In Vivo Fast Ultrasound Super-Resolution Imaging using Nanodroplets
| Autor: | Peter D. Weinberg, Christopher Dunsby, Kirsten Christensen-Jeffries, Robert J. Eckersley, Jacob J. Broughton-Venner, Jiaqi Zhu, Jemma Brown, Meng-Xing Tang, Sevan Harput, Ge Zhang, Matthieu Toulemonde, Kai Riemer |
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| Rok vydání: | 2019 |
| Předmět: |
Diffraction
Materials science business.industry Ultrasound Acoustic wave 01 natural sciences Superresolution 030218 nuclear medicine & medical imaging 03 medical and health sciences 0302 clinical medicine In vivo 0103 physical sciences Microscopy Microbubbles business 010301 acoustics Image resolution Biomedical engineering |
| Zdroj: | 2019 IEEE International Ultrasonics Symposium (IUS). |
| DOI: | 10.1109/ultsym.2019.8926069 |
| Popis: | Current localization-based super-resolution ultrasound imaging requires a low concentration of flowing microbubbles to visualize microvasculature beyond the diffraction limit and acquisition is slow. Nanodroplets offer a promising solution as they can be sparsely activated and deactivated on-demand. In this study, acoustic wave sparsely-activated localization microscopy (AWSALM) using activation and deactivation of nanodroplets, an acoustic counterpart of photo-activated localization microscopy (PALM) which is less dependent on agent concentration and the presence of flow, is demonstrated for super-resolution imaging in deep tissues in vivo. An in vivo super-resolution image of a rabbit kidney is obtained in 1.1 seconds using AWSALM, where micro-vessels with apparent sizes far below the half-wavelength of 220 µm were visualized. This preliminary result demonstrates the feasibility of applying AWSALM for in vivo super-resolution imaging. |
| Databáze: | OpenAIRE |
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