Chromatographic determination of morpholine and products of its microbiological degradation
| Autor: | N. G. Vinokurova, N. F. Zelenkova, A. A. Leont’evskii, I. T. Ermakova, Boris P. Baskunov |
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| Rok vydání: | 2009 |
| Předmět: |
chemistry.chemical_compound
Thiomorpholine Chromatography Column chromatography chemistry Aqueous normal-phase chromatography Hydrophilic interaction chromatography Ion chromatography Supercritical fluid chromatography Reversed-phase chromatography High-performance liquid chromatography Analytical Chemistry |
| Zdroj: | Journal of Analytical Chemistry. 64:618-622 |
| ISSN: | 1608-3199 1061-9348 |
| DOI: | 10.1134/s1061934809060148 |
| Popis: | A combination of thin-layer chromatography (TLC) with high-performance liquid chromatography (HPLC) was shown to be efficient in determining the intermediate products of the utilization of thiomorpholine with ligninolytic basidiomycete fungus Bjerkandera adusta VKM F-3477. The chromatographic mobility of the products of microbiological degradation of thiomorpholine was studied on Sorbfil PTSKh-P-V plates in the systems of chloroform-methanol-25% aqueous ammonia (80: 20: 2) for determining cyclic amines and isopropanol-25% aqueous ammonia (70: 30) for determining thio acids. The optimum conditions were selected for the separation of thiomorpholine and the formed metabolites by ion-exchange chromatography and reversed-phase chromatography. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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