| Popis: |
Pollen of selected Witloof, Robin and Treviso chicory genotypes was isolated and cultured in a modified MS medium supplemented with 0.5 mg/l 2,4-dichlorophenoxy-acetic acid (2,4-D), 0.5 mg/1 indole-3-acetic acid (IAA) and 2.0 mg/l zeatin (Z). During culture periods up to many (6–12) months, the pollen cells emerged and divided, were transferred to MS medium + 0.5 mg/l IAA and 0.5 mg/l 6-benzylaminopurine (BA) for further callus growth. Shoots were induced on the calli following transfer to a low salt medium containing 0.2 mg/l IAA + 0.4 mg/l kinetin (KN); shoots were rooted on a half-concentrated Lepoivre medium + 0.2 mg/l indole-3-butyric acid (IBA). The plants were maintained either in vitro or transferred ex vitro to soil. A range of ploidy levels was measured by DNA fluorescence analysis via flow cytometry in the microspore-derived progeny: some plants were predominantly haploid in a diploid background Genetic fingerprinting by RAPD markers of some of these plants revealed clear differences compared to the parent plants. |
