ChiralN-Substituted Perylene-3,4-dicarboximides as Fluorescent Labeling Reagents

Autor:	Kazumasa Funabiki, Masaki Matsui, Ken-ichi Nakaya, Katsuyoshi Shibata
Rok vydání:	2001
Předmět:	Detection limit General Chemistry Ibuprofen High-performance liquid chromatography chemistry.chemical_compound chemistry Reagent medicine Racemic mixture Enantiomer Acetonitrile Perylene medicine.drug Nuclear chemistry
Zdroj:	Bulletin of the Chemical Society of Japan. 74:549-554
ISSN:	1348-0634 0009-2673
DOI:	10.1246/bcsj.74.549
Popis:	Chiral N-substituted perylene-3,4-dicarboximides have been synthesized. These optically pure compounds were sufficiently soluble in acetonitrile used as fluorescent labeling reagents. Their excitation and emission maxima were observed at λ 500 and 550 nm in acetonitrile, respectively. Enantiomers in a racemic mixture could be analyzed by these reagents. Racemic Ibuprofen and alanine methyl ester were nicely separated by (R)-N-(2-hydroxy-1-phenylethyl)- and (R)-N-(α-carboxyphenethyl)perylene-3,4-dicarboximides in HPLC, respectively. The detection limits (S/N > 3) of Ibuprofen and alanine methyl ester were 1 and 13 pmol, respectively.
Databáze:	OpenAIRE
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