Effect of lectins from Azospirillum brasilense to peroxidase and oxalate oxidase activity regulation in wheat roots
| Autor: | V. E. Nikitina, S. A. Alen’kina |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
biology
Oxalate oxidase Oxalic acid Oxalate oxidase activity Lectin Azospirillum brasilense biology.organism_classification General Biochemistry Genetics and Molecular Biology Enzyme assay Microbiology chemistry.chemical_compound chemistry Biochemistry biology.protein General Agricultural and Biological Sciences Hydrogen peroxide Peroxidase |
| Zdroj: | Biology Bulletin. 37:89-92 |
| ISSN: | 1608-3059 1062-3590 |
| DOI: | 10.1134/s1062359010010127 |
| Popis: | Lectins were extracted from the surface of nitrogen-fixing soil bacteria Azospirillum brasilense Sp7 and from its mutant A. brasilense Sp7.2.3 defective in lectin activity. The ability of lectins to stimulate the rapid formation of hydrogen peroxide related to increase of oxalate oxidase and peroxidase activity in the roots of wheat seedlings has been demonstrated. The most rapid induced pathway of hydrogen peroxide formation in the roots of wheat seedlings was the oxalic acid oxidation by oxalate oxidase which is the effect of lectin in under 10 min in a concentration of 10 μg/ml. The obtained results show that lectins from Azospirillum are capable of inducing the adaptation processes in the roots of wheat seedlings. |
| Databáze: | OpenAIRE |
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