Immunolocalization of Alpha-Actinin in Adult Chicken Skeletal Muscles

Autor: Margaret A. Goldstein, D. B. Zimmer
Rok vydání: 1987
Předmět:
Zdroj: Journal of Electron Microscopy Technique. 6:357-366
ISSN: 1553-0817
0741-0581
DOI: 10.1002/jemt.1060060406
Popis: Immunoelectron microscopy techniques were used to localize alpha-actinin within the Z lattice of adult skeletal muscles. Analysis of electron micrographs by direct visualization demonstrated that anti-alpha-actinin Fab fragments bound throughout the Z lattice. A low-resolution scanning densitometry technique was developed to quantitate the visual increase in the density of the Z lattice. These techniques did not allow determination of the particular component of the Z lattice, amorphous matrix, axial filaments, or cross-connecting filaments with which the antibody was associated. Therefore, additional techniques, including direct measurement of filament diameters and optical diffraction, were utilized in determining which components of the Z lattice bound anti-alpha-actinin Fab fragments. These analyses suggest that the antibody binding is distributed evenly throughout the lattice, along the filaments, and between them and is confined to the region of double overlap of the ends of the thin filaments.
Databáze: OpenAIRE
Externí odkaz: