Mechanical and in vitro characterisation of decellularised porcine aortic tissue conjugated with gold nanoparticles as a vascular repair material
| Autor: | David A. Grant, Allison M. Ostdiek, Sheila A. Grant |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Materials science
Biocompatibility Repair material Biomedical Engineering Nanotechnology Conjugated system medicine.disease Atomic and Molecular Physics and Optics In vitro Biomaterials Colloidal gold Cell culture Aortic tissue medicine Physical and Theoretical Chemistry Calcification Biomedical engineering |
| Zdroj: | International Journal of Nano and Biomaterials. 6:1 |
| ISSN: | 1752-8941 1752-8933 |
| Popis: | There is currently a need for better vascular and cardiac repair materials. These materials must be biocompatible and resist rupture, calcification, and degradation. Materials of biological origin such as decellularised tissue have shown the most promise for achieving these characteristics; however, they still suffer from poor cellular integration and mechanical weakness as compared to the native structures. This study investigated the effects of decellularisation, crosslinking, and conjugation with gold nanoparticles (AuNP) on porcine aortic tissue for use as a vascular repair material. Histology and scanning electron microscopy were performed and confirmed the removal of immunogenic nuclear remnants, confirmed little change in structure after decellularisation, and confirmed the presence of the AuNPs on the material. Mechanical testing was performed which indicated no significant differences in mechanical properties between the native vessel and the modified vessels. Cell culture studies were performed which demonstrated that the modified material was biocompatible. This study confirmed that the decellularised scaffolds maintain the important native tissue characteristics and that crosslinking and the addition of AuNPs achieves biocompatible tissue scaffolds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|