Studies on avian erythrocyte metabolism—XV. Relationship between the major phosphorylated metabolic intermediates and whole blood oxygen affinity in the Gruiformes (cranes)

Autor: D.R. Harkness, M.S. Putman, P.H. Goldman, C.M. Mirande, R.E. Isaacks, C.Y. Kim
Rok vydání: 1987
Předmět:
Zdroj: Comparative Biochemistry and Physiology Part B: Comparative Biochemistry. 87:165-169
ISSN: 0305-0491
Popis: 1. 1. The organic phosphate composition of the red blood cells (RBC), hematological measurements, and hemoglobin functional studies have been determined in fourteen of fifteen species of cranes. 2. 2. The total acid-soluble phosphates (TPi) from the RBC of the cranes ranged from 27.5 μmoles/cm 3 RBC in the Common crane to 39.9 μmoles/cm 3 RBC in the Lesser Sandhill crane. 3. 3. ATP is present at a concentration of 2.1 to 4.0 mM and represents 21.8% of the RBC phosphate in the Eastern Sarus and 30.1% in the Lesser Sandhill crane. 4. 4. The major compound in the red cell of the mature cranes is inositol pentakis phosphate (inositol-P 5 ) ranging from 48% in the Whooping crane to 67% in the Gray-crowned crane. 5. 5. The packed cell volume (PCV) ranges from 37.8 to 46.5%; the hemoglobin concentration ranges from 14.2 to 16.7 g/dl; the red cell count ranges between 1.86 and 2.70 × 10 6 / mm 3 ; the mean cell volumes are unusually large from 213 to 271 μM 3 , P 50 varies from 34.3 to 39.0 mm Hg among the species, and the molar ratios of inositol-P 5 to hemoglobin is 0.54 to 0.83. 6. 6. All species have one hemoglobin component with a mobility relative to human hemoglobin A, ranging between 0.47 to 0.62. 7. 7. In vitro studies indicate that 2,3-bisphosphoglycerate (2,3-P 2 -glycerate), ATP, and inositol-P 5 all modulate hemoglobin function in the species studied.
Databáze: OpenAIRE