Induction of Endothelial Tissue Factor by Endotoxin and Its Precursors

Autor:	Paul D. Rick, Katherine Staskus, C. F. Moldow, Ronald R Bach
Rok vydání:	1993
Předmět:	Messenger RNA Obligate Lipopolysaccharide Monophosphoryl Lipid A Hematology Biology Umbilical vein Cell biology Lipid A Endothelial stem cell Tissue factor chemistry.chemical_compound Biochemistry chemistry lipids (amino acids peptides and proteins)
Zdroj:	Thrombosis and Haemostasis. 70:702-706
ISSN:	2567-689X 0340-6245
DOI:	10.1055/s-0038-1649653
Popis:	SummaryThe structural determinants of lipopolysaccharide required for the induction of tissue factor in human umbilical vein endothelial cells were studied. Intact lipid A was essential for the induction of tissue factor whereas the incomplete lipid A precursors lipid IVA and lipid X, as well as monophosphoryl lipid A and acyloxyacyl hydrolase-treated lipopolysaccharide, were unable to induce tissue factor and tissue factor specific mRNA. However, the lipid A precursor, lipid IVA, was able to inhibit LPS-mediated induction of tissue factor; structural determinants distal to lipid A were found to be required for maximal induction of tissue factor activity and tissue factor mRNA. The presence of serum in the assay was found to amplify but was not obligate for tissue factor induction by LPS.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::afafbc0eb4eb54edd651365cfe5e6452 https://doi.org/10.1055/s-0038-1649653 Zobrazit plný text záznamu