MICROPROPAGATION OFBuddleja cordataAND THE CONTENT OFVERBASCOSIDE AND TOTAL PHENOLS WITH ANTIOXIDANT ACTIVITY OFTHE REGENERATED PLANTLETS
| Autor: | Antonio Bernabé-Antonio, Fernando Rivera-Cabrera, M. E. Estrada-Zúñiga, L. Buendía-González, Rayn Clarenc Aarland, Francisco Cruz-Sosa |
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| Rok vydání: | 2016 |
| Předmět: |
010405 organic chemistry
General Chemical Engineering food and beverages Biology biology.organism_classification 01 natural sciences Buddleja cordata 0104 chemical sciences 010404 medicinal & biomolecular chemistry chemistry.chemical_compound Murashige and Skoog medium Verbascoside Micropropagation chemistry Botany Shoot Gallic acid Gibberellic acid Explant culture |
| Zdroj: | Revista Mexicana de Ingeniería Química. 15:333-346 |
| ISSN: | 2395-8472 1665-2738 |
| DOI: | 10.24275/rmiq/bio1003 |
| Popis: | Buddleja cordata is a medicinal plant distributed in Mexican territory that is characterized for producing phenolic compounds possessing antioxidant activity. It was evaluated the type of morphogenetic responses induced by plant growth regulators. Furthermore, the content of verbascoside and total phenols was determined, as well as the antioxidant activity in regenerated plantlets. The greatest shoot proliferation (29.2 shoots per explant) was achieved in the stem-nodes that were grown in a half-strength Murashige and Skoog medium containing 4.44 M N6-benzyladenine. The greatest shoot heights of 4.3 and 4.7 cm (statistically not dierent) were obtained with gibberellic acid at 4.34 and 8.67 M, respectively. The highest percentage of rooting (89.4%) occurred with 2.45-M indole-3-butyric acid with 20.3 roots per shoot and an average root length of 4.4 cm. Of the rooted shoots, 91.7% were able to survive after 30 days of acclimatization. The verbascoside (1.0 mg g1) and total phenols (24.8 mg of gallic acid equivalents g1) content was related to antioxidant activity of the regenerated plantlets. The micropropagation of B. cordata might represent an alternative about massive production and depict the basis of the establishment of commercial crops and genetic studies. |
| Databáze: | OpenAIRE |
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