Multilayered composite coatings of titanium dioxide nanotubes decorated with zinc oxide and hydroxyapatite nanoparticles: controlled release of Zn and antimicrobial properties against Staphylococcus aureus
| Autor: | Richard D. Handy, Huirong Le, Christopher Tredwin, Alexandros Besinis, Urvashi Fowdar Gunputh |
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| Rok vydání: | 2019 |
| Předmět: |
Composite number
Biophysics Pharmaceutical Science chemistry.chemical_element Bioengineering 02 engineering and technology Zinc engineering.material 010402 general chemistry Cell morphology 01 natural sciences Biomaterials chemistry.chemical_compound Coating Drug Discovery Nanoporous Chemistry Organic Chemistry General Medicine 021001 nanoscience & nanotechnology Antimicrobial Controlled release 0104 chemical sciences Titanium dioxide engineering 0210 nano-technology Nuclear chemistry |
| Zdroj: | International Journal of Nanomedicine. 14:3583-3600 |
| ISSN: | 1178-2013 |
| Popis: | Purpose: This study aimed to decorate the surface of TiO2 nanotubes (TiO2 NTs) grown on medical grade Ti-6Al-4V alloy with an antimicrobial layer of nano zinc oxide particles (nZnO) and then determine if the antimicrobial properties were maintained with a final layer of nano-hydroxyapatite (HA) on the composite. Methods: The additions of nZnO were attempted at three different annealing temperatures: 350, 450 and 550 °C. Of these temperatures, 350°C provided the most uniform and nanoporous coating and was selected for antimicrobial testing. Results: The LIVE/DEAD assay showed that ZnCl2 and nZnO alone were >90% biocidal to the attached bacteria, and nZnO as a coating on the nanotubes resulted in around 70% biocidal activity. The lactate production assay agreed with the LIVE/DEAD assay. The concentrations of lactate produced by the attached bacteria on the surface of nZnO-coated TiO2 NTs and ZnO/HA-coated TiO2 NTs were 0.13±0.03 mM and 0.37±0.1 mM, respectively, which was significantly lower than that produced by the bacteria on TiO2 NTs alone, 1.09±0.30 mM (Kruskal-Wallis, P |
| Databáze: | OpenAIRE |
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