Genome size, heterochromatin organisation, and ribosomal gene mapping in four species of Ribes
| Autor: | Jean-Claude Leclerc, Joëlle Chiche, Spencer Brown, Sonja Siljak-Yakovlev |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
0106 biological sciences
0303 health sciences biology Heterochromatin Chromosome Plant Science Ribes Ribosomal RNA biology.organism_classification 01 natural sciences Nuclear DNA 03 medical and health sciences Ribes alpinum Gene mapping Botany Genome size 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Canadian Journal of Botany. 81:1049-1057 |
| ISSN: | 0008-4026 |
| Popis: | Four wild Ribes species (Ribes alpinum L., Ribes petraeum Wulf., Ribes rubrum L., and Ribes uva- crispa L.; all 2n = 2x = 16) were surveyed for their chromosome and genome organisation. Their genome size was assessed using flow cytometry. Ribes alpinum had 5.3% more nuclear DNA than did the three other species, whose average was 2C = 1.91 pg with 40.4% GC. In addition, GC- and AT-rich heterochromatin and rDNA (18S5.8S26S and 5S) patterns were studied using fluorochrome banding and double-target fluorescence in situ hybridization (FISH), respectively. Only GC-rich heterochromatin was detected, co-localizing with 18S26S rDNA. Fluorochrome banding and FISH patterns revealed marked differences between species. Ribes alpinum and R. uva-crispa differed from R. rubrum and R. petraeum by the number of 18S26S sites and the localization of 5S rDNA. Ribes alpinum and R. uva-crispa were differentiated by the number of 5S sites. Ribes rubrum and R. petraeum also differed by the number of 5S sites and by the size of the GC-rich band on the satellite chromosome pair. These results should contribute to a better understanding of phylogenetic relationships among these species.Key words: Ribes, flow cytometry, fluorochrome banding, FISH, rDNA, NORs. |
| Databáze: | OpenAIRE |
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