Phosphorus Imaging as a Tool for Studying the pH Metabolism in Living Insects

Autor: William A. Laing, Paul T. Callaghan, J.T. Christeller, C.D. Eccles, U. Skibbe
Rok vydání: 1995
Předmět:
Zdroj: Journal of Magnetic Resonance, Series B. 108:262-268
ISSN: 1064-1866
DOI: 10.1006/jmrb.1995.1130
Popis: Comparative 31 P NMR and 1 H NMR imaging experiments at submillimeter pixel resolution were carried out, using a specially constructed solenoidal RF coil. Chemical-shift imaging is used to provide pH maps from the midgut of a Lepidopteran larvae and to demonstrate physiological dependence in the resulting images, The titration curve of pH versus chemical shift for inorganic phosphate is extended beyond the "normal" biological range to the strong alkaline limit.
Databáze: OpenAIRE