| Autor: |
Antonio Foresi, Giuseppina Bertorelli, Dario Olivieri, R. Testi, V. Bocchino, Alfredo Chetta, M. Del Donno, X. Zhuo |
| Rok vydání: |
1998 |
| Předmět: |
|
| Zdroj: |
Clinical & Experimental Allergy. 28:551-560 |
| ISSN: |
0954-7894 |
| Popis: |
Background and objective Antigen processing determines the production of peptides from antigens — including allergens — and their binding to class II major histocompatibility complex molecules, that stimulate T-cell responses. Heat shock protein (hsp) 70 are recognized to have a role in chaperoning antigenic peptides and in facilitating class II peptide assembly. We studied the HLA-DR and hsp70 expression on BAL cells and bronchial biopsies from asthmatics, as well as the effect of low dose fluticasone propionate treatment. Methods Twenty-three asthmatics and eight normal subjects were selected. In each subject BAL and bronchial biopsies were performed. Eighteen out of 23 asthmatics, underwent the second bronchoscopy after 6 weeks of low dose inhaled fluticasone propionate treatment (250 μg bd) in a placebo-controlled double-blind study. BAL fluid and biopsies were processed to evaluate HLA-DR and hsp70 expression by immunochemistry methods. Results Hsp70 and HLA-DR upregulation was present on professional and non-professional antigen presenting cells (APCs). In asthmatics, the hsp70 and HLA-DR expression was higher in BAL (hsp70 P |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
