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Summary. Aim of the study. Development and implementation of colon anastomoses formation method to improve their mechanical strength and biological hermeticity. Materials of the study. A comparative morphological study of the original and traditional two-row colonic anastomoses was performed in the experiment on 18 rabbits, and an assessment of their mechanical strength and biological hermeticity. The assessment of mechanical strength was defined by using a pneumopression technique. The assessment of biological hermeticity was defined by the method of culturing flushing water from the area of the anastomosis to the growth medium, species identification of isolated microorganisms using test systems and calculating the number of colony-forming units by quantitative method. Results of the research. The mechanical strength of one-row manual intra-nodular colonic anastomosis is higher by 66.3–85.4 %, respectively, of the first and seventh day of observations compared with the two-row manual anastomosis. During biological hermeticity assessment in the experimental group, in which colonic anastomosis was performed in the original method, the cultures were sterile at all stages of the sampling, whereas in the control group, where the traditional two-row manual anastomosis was formed, only from the seventh day all the cultures were sterile. Conslusions. The mechanical strength of one-row manual intra-nodular colonic anastomosis using atraumatic suture 4/0 without suturing the mucosa is higher by 66.3-85.4 %, respectively, of the first and seventh day of observations compared with the two-row manual anastomosis. Biological hermeticity of one-row manual intra-nodular colonic anastomosis is 100 % compared with the two-row manual anastomosis (28,5 %). |
