The Effect of Allopurinol on NMDA Receptor Characteristics in the Brain of Fetal Guinea Pigs Following In Utero Hypoxia and Recovery • 214
| Autor: | Anli Zhu, Om P Mishra, Peter J Marro, Maria Delivoria-Papadopoulos, Joanna Kubin |
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| Rok vydání: | 1998 |
| Předmět: | |
| Zdroj: | Pediatric Research. 43:39-39 |
| ISSN: | 1530-0447 0031-3998 |
| Popis: | The present study tests the hypothesis that maternal treatment with allopurinol following in utero hypoxia will attenuate hypoxia induced alterations of the NMDA receptor in the fetal guinea pig brain during recovery. Twenty-eight pregnant guinea pigs at term were studied. Fetuses from normoxic (n=9) and hypoxia (n=7) animals were compared to fetuses from untreated (n=7) and allopurinol treated (n=5) animals exposed to hypoxia but allowed to recover in utero for 24 hours. Maternal hypoxia was induced by lowering inspired oxygen to 7% for 1 hour. Following hypoxia the treated group immediately received 600 mg p.o allopurinol prior to recovery. Brain cell membrane Na+, K+ -ATPase activity was measured as an index of brain cell membrane function. Na+, K+ -ATPase activity was lower in the hypoxic group compared to control (40.7 ± 5.2 vs 29.5 ± 5.2 mmoles Pi/mg protein/hr, P 0.05) indicating increased receptor affinity following hypoxia and recovery. However, the Kd of the allopurinol treated recovery group was similar to control (6.11±0.76 nM). The data shows that allopurinol treatment following in utero hypoxia attenuates hypoxia induced alterations in the NMDA receptor characteristics of fetal guinea pigs during recovery. We speculate that alterations in the NMDA receptor following in utero hypoxia can be modified during recovery by treatment with allopurinol and may prevent delayed brain cell injury. |
| Databáze: | OpenAIRE |
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