Inhibiting Iron Mobilization from Bacterioferritin in Pseudomonas aeruginosa Impairs Biofilm Formation Irrespective of Environmental Iron Availability
| Autor: | Josephine R. Chandler, Huili Yao, Mario Rivera, Anabel Soldano |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
inorganic chemicals
0301 basic medicine biology Pseudomonas aeruginosa Chemistry 030106 microbiology Biofilm Iron deficiency Bacterioferritin medicine.disease_cause biology.organism_classification medicine.disease Cell biology 03 medical and health sciences Cytosol 030104 developmental biology Infectious Diseases medicine biology.protein Bacteria Intracellular Ferredoxin |
| Zdroj: | ACS Infectious Diseases. 6:447-458 |
| ISSN: | 2373-8227 |
| DOI: | 10.1021/acsinfecdis.9b00398 |
| Popis: | Although iron is essential for bacteria, the nutrient presents problems of toxicity and solubility. Bacteria circumvent these problems with the aid of iron storage proteins where Fe3+ is deposited and, when necessary, mobilized as Fe2+ for metabolic requirements. In Pseudomonas aeruginosa, Fe3+ is compartmentalized in bacterioferritin (BfrB), and its mobilization as Fe2+ requires specific binding of a ferredoxin (Bfd) to reduce the stored Fe3+. Blocking the BfrB-Bfd complex leads to irreversible iron accumulation in BfrB and cytosolic iron deprivation. Consequently, given the intracellular iron sufficiency requirement for biofilm development, we hypothesized that blocking the BfrB-Bfd interaction in P. aeruginosa would impair biofilm development. Our results show that planktonic and biofilm-embedded cells where the BfrB-Bfd complex is blocked exhibit cytosolic iron deficiency, and poorly developed biofilms, even in iron-sufficient culture conditions. These results underscore inhibition of the BfrB-Bfd complex as a rational target to dysregulate iron homeostasis and possibly control biofilms. |
| Databáze: | OpenAIRE |
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