Reduced skeletal muscle oxidative capacity and elevated ceramide but not diacylglycerol content in severe obesity
| Autor: | Maja Stefanovic-Racic, John J. Dubé, Vladimir B. Ritov, Elizaveta V. Menshikova, E.M. Leachman, Paul M. Coen, Frederico G.S. Toledo, Kazanna C. Hames, Bret H. Goodpaster, James P. DeLany |
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| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Ceramide Nutrition and Dietetics Triglyceride Endocrinology Diabetes and Metabolism Medicine (miscellaneous) Skeletal muscle medicine.disease Sphingolipid chemistry.chemical_compound Endocrinology Insulin resistance medicine.anatomical_structure Lipotoxicity chemistry Internal medicine Cardiolipin medicine lipids (amino acids peptides and proteins) Diacylglycerol kinase |
| Zdroj: | Obesity. 21:2362-2371 |
| ISSN: | 1930-7381 |
| DOI: | 10.1002/oby.20381 |
| Popis: | Objective The link between a reduced capacity for skeletal muscle mitochondrial fatty acid oxidation (FAO) and lipotoxicity in human insulin resistance has been the subject of intense debate. The objective of this study was to investigate whether reduced FAO is associated with elevated acyl CoA, ceramide, and diacylglycerol (DAG) in severely obese insulin resistant subjects. Methods Muscle biopsies were conducted in lean (L, 22.6 ± 0.5 kg/m2, n = 8), Class I (CI, 32.1 ± 0.4 kg/m2, n = 7) and Class II&III obese (CII&III, 45.6 ± 1.1 kg/m2, n = 15) women for acyl CoA, sphingolipid and DAG profiling. Intramyocellular triglyceride (IMTG) content was determined by histology. FAO was assessed by incubating muscle homogenates with [1-[14]C]palmitate and measuring [14]CO2 production. Cardiolipin content was quantified as an index of mitochondrial content. Lipid metabolism proteins, DGAT1, PLIN5, and PNPLA2 were quantified in biopsy samples by western blot. Results CII&III were more insulin resistant (HOMA-IR: 4.5 ± 0.5 vs. 1.1 ± 0.1, P < 0.001), and had lower FAO (∼58%, P = 0.007) and cardiolipin content (∼31%, P = 0.013) compared to L. IMTG was elevated in CI (P = 0.04) and CII&III (P = 0.04) compared to L. Sphingolipid content was higher in CII&III compared to L (13.6 ± 1.1 vs. 10.3 ± 0.5 pmol/mg, P = 0.031) whereas DAG content was not different among groups. DGAT1 was elevated in CII&III, and PLIN5 was elevated in CI compared to L. Conclusions Severe obesity is associated with reduced muscle oxidative capacity and occurs concomitantly with elevated IMTG, ceramide and insulin resistance. |
| Databáze: | OpenAIRE |
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