The Use of the Chorioallantoic Membrane (CAM) of the Embryonic Chick for the Direct Assessment of Implant Toxicity

Autor:	D.P. Knight, W.S. Pringle, J.R. Shearer, P.P. Monro, D.M. Fyfe
Rok vydání:	1985
Předmět:	Medical Laboratory Technology Chorioallantoic membrane Chemistry Direct assessment Toxicity General Medicine Embryonic chick Implant Toxicology General Biochemistry Genetics and Molecular Biology Cell biology
Zdroj:	Alternatives to Laboratory Animals. 13:261-266
ISSN:	2632-3559 0261-1929
DOI:	10.1177/026119298501300403
Popis:	The toxicity of implant materials requires investigation prior to clinical use. We have developed a method where materials are directly applied to the chorioallantoic membrane (CAM) of 9-day-old chick embryos and toxicity is assessed using histological criteria. We evaluated the method using metal foils. The number and organisation of fibroblasts seemed to be the most useful criteria for assessing metal toxicity. Differences were greatest after 10 days of culture on the CAM. The method is sensitive enough to enable us to discriminate between the less toxic aluminium and titanium and the highly toxic cobalt, nickel and tungsten. The proposed method has advantages over in vitro techniques which provide an abnormal fluid environment and in which the more complex interactions that are possible between implant materials and tissue in vivo cannot be modelled.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::af20bbe0eb9582e71baf2a21da590628 https://doi.org/10.1177/026119298501300403 Zobrazit plný text záznamu