Tide-related Changes in mRNA Abundance of Aromatases and Estrogen Receptors in the Ovary and Brain of the Threespot Wrasse Halichoeres trimaculatus
| Autor: | Shingo Udagawa, Akihiro Takemura, Dae-Ju Oh, Sung-Pyo Hur, Selma Bouchekioua, Yuki Takeuchi, Se-Jae Kim, Ji-Gweon Park, Mathilakath M. Vijayan, Thomas W. Moon, Neelakanteswar Aluru, Yong-Ju Park |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty biology Estrogen receptor 030209 endocrinology & metabolism Ovary Oceanography biology.organism_classification 03 medical and health sciences Gonadosomatic Index 030104 developmental biology 0302 clinical medicine Endocrinology medicine.anatomical_structure Wrasse Internal medicine Forebrain medicine Vitellogenesis Development of the gonads Testosterone |
| Zdroj: | Ocean Science Journal. 53:239-249 |
| ISSN: | 2005-7172 1738-5261 |
| Popis: | The threespot wrasse (Halichoeres trimaculatus; Family Labridae) is a common coral reef species of the Indo-Pacific Ocean. Given that this species spawns daily at high tide (HT), we hypothesized that endocrine changes in relation to gonadal development are synchronized with the tidal cycle. To test this, we examined the transcript abundance of two cytochrome P450 aromatases (cyp19a and cyp19b) and two estrogen receptors (erα and erβ) in the ovary and brain of this species in response to tidal change. When fish were collected around four tidal points [low tide (LT), flood tide (FT), high tide (HT), and ebb tide (ET)], gonadosomatic index and oocyte diameter increased around HT and FT, respectively. Ovulatory follicles were observed in ovaries around HT. Real-time quantitative polymerase-chain reaction revealed that mRNA abundance of cyp19a and erα, but not erβ, in the ovary increased around ET and HT, respectively. On the other hand, mRNA levels of cyp19b in the forebrain were significantly higher around FT. Increases of erα and erβ mRNA abundance around FT were observed in all areas of the brain and the midbrain, respectively. The changes in mRNA abundance of key genes involved in reproduction at specific tidal cycles, along with the development of the vitellogenic oocytes in the ovary, support our hypothesis that synchronization of endocrine changes to the tidal periodicity plays a role in the gonadal development of this species. We hypothesize that conversion of testosterone to E2 in the brain may be associated with the spawning behavior given that the wrasse exhibits group spawning with a territory-holding male around HT. |
| Databáze: | OpenAIRE |
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