Enhanced β-carotene production by Rhodotorula glutinis using high hydrostatic pressure

Autor:	Jun-She Sun, Sui-Lou Wang, Xiao-Zong Wu, Bei-Zhong Han
Rok vydání:	2008
Předmět:	Strain (chemistry) biology General Chemical Engineering medicine.medical_treatment Hydrostatic pressure Carotene Mutant General Chemistry Gene mutation Rhodotorula biology.organism_classification chemistry.chemical_compound Biochemistry Biosynthesis chemistry Nucleic acid medicine Food science
Zdroj:	Korean Journal of Chemical Engineering. 25:513-516
ISSN:	1975-7220 0256-1115
DOI:	10.1007/s11814-008-0086-2
Popis:	High hydrostatic pressure (HHP) technology was used for improving the ability of β-carotene biosynthesis of Rhodotorula glutinis R68. After the treatments of five repeated cycles at 300 MPa for 15 min, the barotolerant mutant PR68 was obtained. After 72 h of culture, the biomass of mutant PR68 was 21.6 g/L, decreased by 8.5% compared to the parent strain R68, but its β-carotene production reached 19.4 mg/L, increased by 52.8% compared to the parent strain R68. The result of restriction fragment length polymorphism analysis suggested that mutant strain PR68 was likely to change in nucleic acid level, and thus enhanced β-carotene production in this strain was a result of gene mutation induced by HHP treatment. HHP technology seems a promising approach for improving industrial microbes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::af0bde138a8e70747af30dc4594bf042 https://doi.org/10.1007/s11814-008-0086-2 Zobrazit plný text záznamu Full text from SpringerLink