| Autor: |
Edouard Bertrand, Laura Cuyas, Marie-Christine Thibaud, Eric Maréchal, Nathalie Pochon, Sahar Hani, Florian Mueller, Laurent Nussaume, Hélène Javot, Rémy Merret, James Whelan, Pascale David, Orestis Faklaris, David Secco |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.1101/2021.04.12.439441 |
| Popis: |
Plants are sessile organisms constantly adapting to ambient fluctuations through spatial and temporal transcriptional responses. Here, we implemented the latest generation RNA imaging system and combined it with microfluidics to visualize transcriptional regulation in living Arabidopsis plants. This enabled quantitative measurements of the transcriptional activity of single loci in single cells, real time and changing environmental conditions. Using phosphate responsive genes as model, we found that active genes displayed high transcription initiation rates (∼3s) and frequently clustered together in endoreplicated cells. We observed gene bursting and large allelic differences in single cells, revealing that at steady-state, intrinsic noise dominated extrinsic variations. Moreover, we established that transcriptional repression triggered in roots by phosphate, a crucial macronutrient limiting plant development, occurred with unexpected fast kinetics (∼minutes) and striking heterogeneity between neighboring cells. Access to single cell RNA polymerase II dynamics within live plants will benefit future studies of signaling processes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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