ISOLATION AND IDENTIFICATION OF 6β, 17α, 21-TRIHYDROXY-6α-METHYL-Δ4-PREGNENE-3,20-DIONE (21-ACETATE) FROM THE URINE OF HUMAN SUBJECTS TREATED WITH 6α-METHYL-17α-ACETOXYPROGESTERONE

Autor:	G. Sala, E. Castegnaro
Rok vydání:	1962
Předmět:	medicine.medical_specialty Endocrinology Chromatography Pregnene Isolation (health care) Chemistry Endocrinology Diabetes and Metabolism Internal medicine medicine Urine
Zdroj:	Journal of Endocrinology. 24:445-452
ISSN:	1479-6805 0022-0795
DOI:	10.1677/joe.0.0240445
Popis:	SUMMARY A new tetrazolium reducing compound, present as a conjugate which could be hydrolysed by β-glucuronidase, has been isolated from the urine of normal men and women and oophorectomized-adrenalectomized women, all of whom had received 100 mg./day 6α-methyl-17α-acetoxyprogesterone (MAP) either subcutaneously or orally. Detailed studies of its characteristics, including degradation reactions, ultraviolet and infrared spectra, combined with studies on known steroids, suggest that the new compound is 6β,17α,21-trihydroxy-6α-methyl-Δ4-pregnene-3,20-dione (21-acetate). It is suggested that MAP in vivo undergoes hydroxylation at C-21 in the absence of ovarian and adrenal tissue which up to the present have been considered essential for this metabolic process.
Databáze:	OpenAIRE
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