Bayesian inference associates rare KDR variants with specific phenotypes in pulmonary arterial hypertension
| Autor: | Karyn Megy, William C. Nichols, Chung K. Wendy, Jonathan Stephens, Nicholas W. Morrell, Christopher J. Penkett, Stefan Gräf, Tobias Tilly, Richard C. Trembath, Michael W. Pauciulo, Divya Pandya, Carrie L. Welch, Carmen M. Treacy, Allan Lawrie, Smitha Rajaram, Martin R. Wilkins, Yufeng Shen, Emilia M. Swietlik, Andrew J. Swift, Laura Southgate, Marcella Cogliano, Na Zhu, Heritable Pah, Katie A. Lutz, Daniel Greene, US Pah Biobank Enrolling Centers' Investigators, Jennifer M. Martin |
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| Rok vydání: | 2019 |
| Předmět: |
Genetics
0303 health sciences Candidate gene Kinase insert domain receptor Biology Bayesian inference medicine.disease Phenotype Pulmonary hypertension 3. Good health 03 medical and health sciences 0302 clinical medicine Missing heritability problem 030220 oncology & carcinogenesis Rare mutations medicine Gene 030304 developmental biology |
| DOI: | 10.1101/2019.12.11.871210 |
| Popis: | BackgroundApproximately 25% of patients with pulmonary arterial hypertension (PAH) have been found to harbor rare mutations in disease-causing genes. To identify missing heritability in PAH we integrated deep phenotyping with whole-genome sequencing data using Bayesian statistics.MethodsWe analyzed 13,037 participants enrolled in the NIHR BioResource - Rare Diseases (NBR) study, of which 1,148 were recruited to the PAH domain. To test for genetic associations between genes and selected phenotypes of pulmonary hypertension (PH), we used the Bayesian rare-variant association method BeviMed.ResultsHeterozygous, high impact, likely loss-of-function variants in the Kinase Insert Domain Receptor (KDR) gene were strongly associated with significantly reduced transfer coefficient for carbon monoxide (KCO, posterior probability (PP)=0.989) and older age at diagnosis (PP=0.912). We also provide evidence for familial segregation of a rare nonsense KDR variant with these phenotypes. On computed tomographic imaging of the lungs, a range of parenchymal abnormalities were observed in the five patients harboring these predicted deleterious variants in KDR. Four additional PAH cases with rare likely loss-of-function variants in KDR were independently identified in the US PAH Biobank cohort with similar phenotypic characteristics.ConclusionsThe Bayesian inference approach allowed us to independently validate KDR, which encodes for the Vascular Endothelial Growth Factor Receptor 2 (VEGFR2), as a novel PAH candidate gene. Furthermore, this approach specifically associated high impact likely loss-of-function variants in the genetically constrained gene with distinct phenotypes. These findings provide evidence for KDR being a clinically actionable PAH gene and further support the central role of the vascular endothelium in the pathobiology of PAH. |
| Databáze: | OpenAIRE |
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