Immunoelectron microscopic study of the localization of transferrin receptor in HeLa cells

Autor:	Takao Miyamoto, Kazuhiko Suzuki, Yutaka Kimijima, Shuji Shimada, Nobuyuki Tanaka, Kenichi Shionoya, Teruo Amagasa
Rok vydání:	1993
Předmět:	biology medicine.drug_class Chemistry Vesicle Transferrin receptor Monoclonal antibody biology.organism_classification Cell biology law.invention HeLa Cell membrane medicine.anatomical_structure law Cancer cell medicine Electron microscope Intracellular
Zdroj:	Japanese Journal of Oral & Maxillofacial Surgery. 39:814-818
ISSN:	2186-1579 0021-5163
DOI:	10.5794/jjoms.39.814
Popis:	Immunocytochemical localization of the transferrin receptor (TfR) using a monoclonal antibody in HeLa cells was investigated by microscopy and electron microscopy. In addition, changes in transferrin receptor localization evoked by cisplatinum diammine dichloride (CDDP) were examined. The results were as follows:1. In proliferating cells, TfR was found in the cell membrane and electron microscopic investigations revealed that TfR was localized in a scattered fashion in the cell membrane, microvillus-like projections and intercellular vesicles.2. After CDDP treatment, microvillus-like projections and intercellular vesicles were not as prominent as in the control group and the quantity of TfR decreased.3. In some relatively highly degenerated cells by CDDP, many TfRs were demonstrated, suggesting that the mechanism of TfR expression, which reflects the proliferative ability of cancer cells, should be investigated.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::aef9883f9869ebf5d87a4df2346e27f2 https://doi.org/10.5794/jjoms.39.814 Zobrazit plný text záznamu