The comparison of lectin spectra of certain representatives of the speciesPhaseolus vulgaris ssp.aborigineus of different geographical origin

Autor: Jiřina Švachulová, Eva Klozvá, Věra Turková, Daniela Přikrylová, Věra Hadačová
Rok vydání: 1984
Předmět:
Zdroj: Biologia Plantarum. 26:349-357
ISSN: 1573-8264
0006-3134
DOI: 10.1007/bf02898571
Popis: Using biospecific chromatography on fetuin D-Glc-Separon H 1.000, lectins were isolated from the seeds of six representatives of the speciesPhaseolus vulgaris ssp.aborigineus of different geographical origin. The lectins of all the six representatives exhibit the agglutinating activity against rabbit erythrocytes (non-treated, tryp3in-treated and pronase-treated) as well as against human erythrocytes (irrespective of blood group) but of different quantity. Lectins isolated from 4 seed types showed mitogenic activity against lymphocytes of murine spleen, whereas in two seed types mitogenic activity was not proved. Isoelectric focusing in polyacrylamide gels revealed in the different seed types 2 to 5 bands of lectins within the range of pI 4–6. Differences in the composition of lectin spectra were proved by means of immunoelectrophoresis and double diffusion. The presence of α-D-galactosidase has not been established in any isolated lectin.
Databáze: OpenAIRE
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