Lentil (Lens culinaris L.) growth promoting rhizobacteria and their effect on nodulation in coinoculation with rhizobia
Autor: | Jorge Campos, Macarena Gerding, Ernesto A. Moya-Elizondo, Pía Oyarzúa, Marisol Vargas, Maurine Sepúlveda-Caamaño |
---|---|
Rok vydání: | 2017 |
Předmět: |
0106 biological sciences
Inoculation Pseudomonas food and beverages Soil Science 04 agricultural and veterinary sciences Biology Rhizobacteria 16S ribosomal RNA biology.organism_classification 01 natural sciences Rhizobia Agronomy Seedling 040103 agronomy & agriculture Nitrogen fixation 0401 agriculture forestry and fisheries Agronomy and Crop Science Bacteria 010606 plant biology & botany |
Zdroj: | Archives of Agronomy and Soil Science. 64:244-256 |
ISSN: | 1476-3567 0365-0340 |
DOI: | 10.1080/03650340.2017.1342034 |
Popis: | Lentil is cultivated in Chilean Mediterranean drylands, in areas with soils that are nutrient depleted and eroded. Inoculation of lentil with rhizobia in co-inoculation with growth promoting rhizobacteria would allow higher biomass and an opportunity for early nodulation and increased nitrogen fixation. The objective of this research was to select rhizosferic bacteria (PGPR) from lentils and to evaluate their effect on lentil nodulation in co-inoculation with rhizobia. Sixty three lentil rhizobacteria isolates where obtained from nine soils in the mediterranean area. These were fingerprinted through BOX-PCR reducing the number to 57 distinct strains. The strains were evaluated for ACCdeaminase activity, IAA production and compatibility with rhizobia. Seventeen strains showed ACC-deaminase activity, all of them synthesized IAA and 38 were compatible with the rhizobia. Ten selected strains were identified as Pseudomonas spp. through 16S rRNA sequencing. The strains were inoculated in lentil seedling... |
Databáze: | OpenAIRE |
Externí odkaz: |