Glycosidases Interact Selectively With Mannose-6-Phosphate Receptors of Bull Spermatozoa
| Autor: | Andrea C. Aguilera, Veronica Boschin, Miguel A. Sosa, Juan C. Cavicchia, Lorena Carvelli |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
endocrine system Cell Mannose 6-phosphate Immunofluorescence Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Epididymal fluid Receptor Molecular Biology reproductive and urinary physiology chemistry.chemical_classification 030219 obstetrics & reproductive medicine medicine.diagnostic_test urogenital system Cell Biology Sperm 030104 developmental biology Enzyme medicine.anatomical_structure chemistry Distribution pattern |
| Zdroj: | Journal of Cellular Biochemistry. 117:2464-2472 |
| ISSN: | 0730-2312 |
| DOI: | 10.1002/jcb.25538 |
| Popis: | Glycosidases may play a role in sperm maturation during epididymal transit. In this work, we describe the interaction of these enzymes with bull spermatozoa. We found that β-galactosidase associated to spermatozoa can be released under low ionic strength conditions, whereas the interaction of N-acetyl-β-D-glucosaminidase and β-glucuronidase with spermatozoa appeared to be stronger. On the other hand, α-mannosidase and α-fucosidase cannot be removed from the gametes. In addition, part of N-acetyl-β-D-glucosaminidase, β-galactosidase, and β-glucuronidase can also be released by mannose-6-phosphate. Taking into account these data, we explored the presence of cation-independent- and cation-dependent-mannose-6-phosphate receptors in the spermatozoa and found that cation-independent mannose-6-phosphate receptor is highly expressed in bull spermatozoa and cation-dependent-mannose-6-phosphate receptor is expressed at a lesser extent. In addition, by immunofluorescence, we observed that cation-independent-mannose-6-phosphate receptor is mostly located at the acrosomal zone, whereas cation-dependent-mannose-6-phosphate receptor presents a different distribution pattern on spermatozoa during the epididymal transit. N-acetyl-β-D-glucosaminidase and β-glucuronidase isolated from epididymal fluid interacted mostly with cation-independent-mannose-6-phosphate receptor, while β-galactosidase was recognized by both receptors. We concluded that glycosidases might play different roles in bull spermatozoa and that mannos-6-phosphate receptors may act as recruiters of some enzymes. J. Cell. Biochem. 117: 2464-2472, 2016. © 2016 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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