One-pot synthesis of dual-emitting BSA–Pt–Au bimetallic nanoclusters for fluorescence ratiometric detection of mercury ions and cysteine
| Autor: | Yun-Xia Guo, Shou-Nian Ding |
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| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Analytical Methods. 7:5787-5793 |
| ISSN: | 1759-9679 1759-9660 |
| DOI: | 10.1039/c5ay01112d |
| Popis: | Water-soluble and dual-emitting bovine serum albumin-templated bimetallic platinum–gold fluorescent nanoclusters (BSA–Pt–Au NCs) have been synthesized for the first time by a simple and rapid one-pot synthesis strategy at a vital molar ratio of Pt/Au precursors under basic conditions. The BSA–Pt–Au NCs show unique and well-resolved dual-emission bands at 405 nm and 640 nm corresponding to the emission of the BSA–metal complex and Au NCs under the excitation at 340 nm, respectively. Interestingly, with the addition of mercury(II) (Hg2+) ions, the emission intensity at 405 nm (F405) has a negligible influence, while the emission intensity at 640 nm (F640) decreases dramatically, which is due to the specific and strong d10–d10 interactions between Hg2+ ions and Au atoms/ions on the surface of the BSA–Pt–Au NCs. Meanwhile, cysteine (Cys) could effectively block Hg2+ ions to quench the fluorescence of BSA–Pt–Au NCs, which is based on the strong interaction between Hg2+ ions and the thiol functional group. These allowed the analysis of Hg2+ ions and Cys with the ratio of two emission intensities (F405/F640) in ultra-sensitivity and selectivity with high accuracy. The limit of detection (LOD) was 0.3 nM for Hg2+ ions and 0.04 μM for Cys (S/N = 3), respectively. This work indicates that the fluorescent sensor possesses potential applications for biological detection. |
| Databáze: | OpenAIRE |
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