Rational Design of an Ex Vivo Model of Thrombosis

Autor:	Patrick Simonson, Sandra Rugonyi, Ishan Patel, Andras Gruber, Tara C. White-Adams, Owen J. T. McCarty, Michelle A. Berny
Rok vydání:	2010
Předmět:	Pathology medicine.medical_specialty business.industry medicine.disease Thrombosis General Biochemistry Genetics and Molecular Biology Pathogenesis Thrombin Coagulation Modeling and Simulation Occlusion medicine Platelet Thrombus business Ex vivo medicine.drug
Zdroj:	Cellular and Molecular Bioengineering. 3:187-189
ISSN:	1865-5033 1865-5025
DOI:	10.1007/s12195-010-0103-5
Popis:	The underlying pathogenesis of cardiovascular disease is the formation of occlusive thrombi. While many well-defined animal models recapitulate the process of intravascular thrombosis, there is a need for validated ex vivo models of occlusive thrombus formation. Using the force of gravity to provide a constant pressure gradient, we designed and validated an ex vivo model of thrombosis. Times to occlusion on a collagen matrix in our model were within the range of occlusion times observed in murine thrombosis models. Prolongation of time to occlusion in the presence of platelet αIIbβ3 antagonists or inhibitors to thrombin or activated factor X is in agreement with established mechanisms of thrombus formation. The use of this model may be expanded to characterize the mechanisms of thrombosis and to determine the efficacy of pharmacological agents designed to prevent occlusive thrombus formation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::aec9bf5878f352eeb839b08b0b9f43c8 https://doi.org/10.1007/s12195-010-0103-5 Zobrazit plný text záznamu Full text from SpringerLink