The P2Y14 receptor in the trigeminal ganglion contributes to the maintenance of inflammatory pain
| Autor: | Fei Liu, Liu Mengke, Jiefei Shen, Yan-Yan Zhang, Jiu Lin, Hang Wang, Chao-Lan Huang, Fang Xinyi, Da‐qing Liao, Cheng Zhou |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Orofacial pain medicine.medical_specialty Glial fibrillary acidic protein biology Chemistry Receptor expression Purinergic receptor Cell Biology 03 medical and health sciences Cellular and Molecular Neuroscience Trigeminal ganglion 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Endocrinology Dorsal root ganglion Downregulation and upregulation Internal medicine medicine biology.protein medicine.symptom Receptor 030217 neurology & neurosurgery |
| Zdroj: | Neurochemistry International. 131:104567 |
| ISSN: | 0197-0186 |
| Popis: | P2Y purinergic receptors expressed in neurons and satellite glial cells (SGCs) of the trigeminal ganglion (TG) contribute to inflammatory and neuropathic pain. P2Y14 receptor expression is reported in the spinal cord, dorsal root ganglion (DRG), and TG. In present study, the role of P2Y14 receptor in the TG in inflammatory orofacial pain of Sprague-Dawley (SD) rats was investigated. Peripheral injection of complete Freund's adjuvant (CFA) induced mechanical hyperalgesia with the rapid upregulation of P2Y14 receptor, glial fibrillary acidic protein (GFAP), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), C-C chemokine CCL2, phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK1/2), and phosphorylated p38 (p-p38) proteins in the TG. Furthermore, immunofluorescence staining confirmed the CFA-induced upregulation of P2Y14 receptor. Double immunostaining showed that P2Y14 receptor colocalized with glutamine synthetase (GS) and neuronal nuclei (NeuN). Finally, trigeminal injection of a selective antagonist (PPTN) of P2Y14 receptor attenuated CFA-induced mechanical hyperalgesia. PPTN also decreased the upregulation of the GFAP, IL-1β, TNF-α, CCL2, p-ERK1/2, and p-p38 proteins. Our findings showed that P2Y14 receptor in TG may contribute to orofacial inflammatory pain via regulating SGCs activation, releasing cytokines (IL-1β, TNF-α, and CCL2), and phosphorylating ERK1/2 and p38. |
| Databáze: | OpenAIRE |
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