Cyclic peptides and their interaction with peptide coated surfaces

Autor: V. Hoffmann, Günther Jung, Christiane Stingel, Frauke Palmer, Dietmar Leipert, Rolf Tünnemann
Rok vydání: 2001
Předmět:
Zdroj: Journal of Molecular Structure. :153-157
ISSN: 0022-2860
DOI: 10.1016/s0022-2860(01)00449-5
Popis: Focusing on biochemical and pharmaceutical inhibitor systems the interaction of cyclic peptides with model peptides have been investigated by ATR-FTIR-spectroscopy. Information about the participation of special functional groups e.g. COOH, COO − , NH 3 + or peptide backbone was gathered by observing cyclohexapeptides (c(X 1 LX 2 LX 3 )) which are interacting with covalently coated Si-ATR-crystals ( l -arginine, tripeptide I (aNS), tripeptide II (SNa)). To determine the interaction, further studies about the band sequence (1800–1500 cm −1 ) for non-adsorbed cyclohexapeptides and for the interaction with the silicon surface (SiOH) were necessary. The spectra of the interacting cyclohexapeptides with the SiOH-groups were treated like reference spectra for the evaluation of the peptide–peptide interaction. Based on these spectra, we can conclude that there is peptide–peptide interaction with the coating and not with the residual OH-groups. Determination of interaction mechanisms was done by spectra which represent adsorbed molecules only. The amount of adsorbed molecules was considerably less than a monolayer. Therefore the intensities of the spectra are about 10 −4 absorbance units. The spectra contain information about both changes of the coating and of the cyclohexapeptide.
Databáze: OpenAIRE
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