A truncated Sph12-38 with potent antimicrobial activity showing resistance against bacterial challenge in Oryzias melastigma
| Autor: | Ke-Jian Wang, Dan-Qing Fan, Ying Yang, Yan-Chao Chen, Lin Hou, Xiaowan Ma, Bei Chen |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Gram-negative bacteria 030102 biochemistry & molecular biology biology Gram-positive bacteria Antimicrobial peptides Pseudomonas fluorescens General Medicine Bacillus subtilis Aquatic Science biology.organism_classification Antimicrobial Microbiology 03 medical and health sciences Minimum inhibitory concentration 030104 developmental biology Environmental Chemistry Bacteria |
| Zdroj: | Fish & Shellfish Immunology. 67:561-570 |
| ISSN: | 1050-4648 |
| Popis: | Antimicrobial peptides (AMPs) represent an efficient part of innate immunity and are found in a variety of life. Among them Histone 2A (H2A), as a promising class of AMPs, attracts great attention, but the in vivo mechanism of H2A derived AMP is still less known. Based on the acquisition of Sphistin, a synthetic 38-amino acid H2A derived peptide from Scylla paramamosain , as reported in our previous study, was truncated into three short fragments (Sph 12-38 , Sph 20-38 and Sph 30-38 ) and further investigated for its possible functional domains. The antimicrobial activities of these analogs against different Gram-positive bacteria, Gram-negative bacteria and fungi were illustrated. Among the analogs, Sph 12-38 showed a stronger activity with a much lower minimum inhibitory concentration (3 μM) against Staphylococcus aureus, Corynebacterium glutamicum, Micrococcus lysodeikticus Fleming, Bacillus subtilis, Pseudomonas fluorescens, Aeromonas hydrophila and A. sobria in comparison with the reported Sphistin. A leakage of intracellular content was described in E. coli treated with Sph 12-38 . Unlike Sphistin which mainly disrupts the membrane integrity, Sph 12-38 could also combine the A. sobria genomic DNA with a minimum concentration of 6 μM and was located intracellularly in cells observed under confocal laser scanning microscope imaging. In comparison with the control group of Oryzias melastigma injected with A. sobria alone, the group treated with a mixture of Sph 12-38 and A. sobria showed a higher survival rate 7 days post-injection. Furthermore, in a pretreatment assay at 6 h, a higher survival rate was observed in the group injected with the mixture of Sph 12-38 and A. sobria . Taken together, the synthetic peptide of Sph 12-38 had a potent antimicrobial activity against bacteria. However, Sph 12-38 had no cytotoxicity towards the hemolymph of S. paramamosain . Our study suggested that, as with Sph 12-38 , the H2A derived peptides were more likely prone to exert their activities in vivo through the truncated fragments while defending against different species of pathogens. |
| Databáze: | OpenAIRE |
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