Adsorption of Lysozyme on a Novel Cross-linked Poly(vinyl alcohol) Hydrogel
Autor: | Fukuda Takeshi, Hiroki Bunkyo-ku Degawa, Shirogane Taiichi, Kohhei Tomari, Haruyasu Tanigawa, Yuki Ikeda, Noriko Saitoh, Osamu Watanabe, Kenji Uno |
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Rok vydání: | 1999 |
Předmět: | |
Zdroj: | NIPPON KAGAKU KAISHI. :775-776 |
ISSN: | 2185-0925 0369-4577 |
Popis: | Lysozyme adsorption to cross-linked poly (vinyl alcohol) (c-PVA) hydrogel was studied in vitro. cPVA hydrogel was prepared from saponified copolymers of vinyl acetate and triallyl isocyanurate by exposure to ultraviolet light. In vitro, c-PVA hydrogel and four types of 2-hydroxyethyl methacrylate (HEMA) hydrogel were used. Hydrogels were individually kept in the 0.5 w/v% lysozyme solution at 37°C for 24 hours. The lysozyme adsorbed to hydrogels was extracted with 1 w/v% sodium dodecyl sulfonate (SDS) respectively. The quantity of extracted lysozyme was determined using 2, 2'-bicinchoninic acid. The adsorbed lysozyme of c-PVA hydrogel was much less than those of four types of HEMA hydrogel. These results suggest that our PVA hydrogel may be promising as a biomaterial like soft contactlens. |
Databáze: | OpenAIRE |
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