Adsorption of Lysozyme on a Novel Cross-linked Poly(vinyl alcohol) Hydrogel

Autor:	Fukuda Takeshi, Hiroki Bunkyo-ku Degawa, Shirogane Taiichi, Kohhei Tomari, Haruyasu Tanigawa, Yuki Ikeda, Noriko Saitoh, Osamu Watanabe, Kenji Uno
Rok vydání:	1999
Předmět:	chemistry.chemical_compound Vinyl alcohol Sulfonate chemistry Self-healing hydrogels Polymer chemistry Ultraviolet light Vinyl acetate Copolymer General Chemistry Lysozyme Methacrylate
Zdroj:	NIPPON KAGAKU KAISHI. :775-776
ISSN:	2185-0925 0369-4577
Popis:	Lysozyme adsorption to cross-linked poly (vinyl alcohol) (c-PVA) hydrogel was studied in vitro. cPVA hydrogel was prepared from saponified copolymers of vinyl acetate and triallyl isocyanurate by exposure to ultraviolet light. In vitro, c-PVA hydrogel and four types of 2-hydroxyethyl methacrylate (HEMA) hydrogel were used. Hydrogels were individually kept in the 0.5 w/v% lysozyme solution at 37°C for 24 hours. The lysozyme adsorbed to hydrogels was extracted with 1 w/v% sodium dodecyl sulfonate (SDS) respectively. The quantity of extracted lysozyme was determined using 2, 2'-bicinchoninic acid. The adsorbed lysozyme of c-PVA hydrogel was much less than those of four types of HEMA hydrogel. These results suggest that our PVA hydrogel may be promising as a biomaterial like soft contactlens.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ae9b2e25c831418969faaa24e0a97c19 https://doi.org/10.1246/nikkashi.1999.775 Zobrazit plný text záznamu