| Autor: |
John T. Y. Wu, Lester S. Y. Wong, Eva Y. W. Chow, Evelyn E. Bowlby |
| Rok vydání: |
2003 |
| Předmět: |
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| Zdroj: |
JALA: Journal of the Association for Laboratory Automation. 8:42-50 |
| ISSN: |
1535-5535 |
| DOI: |
10.1016/s1535-5535-03-00008-x |
| Popis: |
The authors automated an enzyme-linked immunosorbent assay to detect porcine serum antibodies to Toxoplasma gondii. Two thousand swine sera can be assayed in two eight-hour shifts using a robotic workstation. The automated-ELISA programming is not complex and the test configuration is flexible. This high-throughput screening (HTS) a-ELISA can achieve a 10-fold increase (100→ 1000 tests) in test capacity over the manual method. The assay has been validated according to the requirements of the ISO/IEC 17025 standard. These include repeatability, reproducibility, and optimal threshold value studies. Other requirements are proficiency panel testing, analyst training, standard operation procedure, and equipment certifications. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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