Autor:	Jacqueline Paly, Phil L'Huillier, Laurence Lepourry, Marie-Georges Stinnakre, Solange Soulier, Jean-Claude Mercier, Jean Djiane, Brett Langley, Jean-Luc Vilotte
Rok vydání:	1999
Předmět:	Genetically modified mouse Reporter gene animal structures Transgene Promoter Biology Molecular biology Prolactin Regulatory sequence In vivo Gene expression Genetics Animal Science and Zoology Agronomy and Crop Science Biotechnology
Zdroj:	Transgenic Research. 8:23-31
ISSN:	0962-8819
DOI:	10.1023/a:1008851802022
Popis:	In order to establish a possible correlation between in vitro prolactin induction and the transcriptional activity of mammary gene promoters in transgenic mice, a functional Stat5-binding site was created by means of site-directed mutagenesis at position −70 on a 560 bp murine α-lactalbumin promotor linked to a CAT reporter gene. Surprisingly, the wild-type promoter was constitutively active in vitro and could not be induced by prolactin. Introducing the proximal Stat5 site abolished this constitutive activity and resulted in prolactin dependence in both CHO-K1- and HC11-transfected cells. In transgenic mice, both the frequency of lines expressing the transgene and the prevalence of mid to late pregnancy expression were increased.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ae8294f0f68d026252e45f2181582240 https://doi.org/10.1023/a:1008851802022 Zobrazit plný text záznamu Full text from SpringerLink