Upregulation of F-actin and ?-actinin in reactive astrocytes

Autor:	Sergey Fedoroff, Ebtesam M. Abd-El-Basset
Rok vydání:	1997
Předmět:	Cellular and Molecular Neuroscience Actinin alpha 1 Tissue culture Downregulation and upregulation biology.protein Motility macromolecular substances Actin-binding protein Biology Microfilament Actin Cell biology
Zdroj:	Journal of Neuroscience Research. 49:608-616
ISSN:	1097-4547 0360-4012
DOI:	10.1002/(sici)1097-4547(19970901)49:5<608::aid-jnr11>3.0.co;2-r
Popis:	We have shown previously that in tissue culture stellate astrocytes downregulate F-actin and actin binding proteins (ABPs) (Abd-El-Basset et al.: J Neurosci Res 30:1-17, 1991), whereas the reactive-like astrocytes upregulate their F-actin (Fedoroff et al.: Neuroscience 22:255-266, 1987). In the present study we report that in normal brain, as in tissue culture, neither F-actin nor alpha-actinin (an ABP) could be detected in stellate astrocytes. When a stab wound was made in brain, F-actin and alpha-actinin were upregulated in reactive astrocytes. We also demonstrated that reactive-like astrocytes in tissue culture express alpha-actinin, which has a "dotted" appearance when immunostained, and is colocalized with F-actin in a specific arrangement.
Databáze:	OpenAIRE
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