Synthesis of butyl butyrate in 93 % yield by Thermomyces lanuginosus lipase on waste eggshells
| Autor: | Yen Shan See, Suhaila Salleh, Noor Aziah Serri, Sufia Hena, Husnul Azan Tajarudin |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Chromatography Immobilized enzyme biology Chemistry Biodegradable waste 01 natural sciences 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Adsorption 010608 biotechnology Yield (chemistry) biology.protein Environmental Chemistry Response surface methodology Eggshell Lipase Butyl butyrate |
| Zdroj: | Environmental Chemistry Letters. 14:189-194 |
| ISSN: | 1610-3661 1610-3653 |
| Popis: | There is a high demand for immobilized enzymes in the industry because it is a green technology, it improves the efficiency of the crude enzyme and it minimizes the step of product recovery. Selection of a suitable carrier for enzyme immobilization is crucial. Eggshells are abundantly available as an organic waste. Here, lipase from Thermomyces lanuginosus was immobilized on eggshell to produce butyl butyrate. We evaluated the effect of lipase loading, from 0.5 to 3.0 %, size of eggshells, lower than 1, 1–2 and 2–4 mm, and temperature, from 30 to 50 °C. Results show that the optimum conditions calculated by response surface methodology analysis for butyl butyrate synthesis were 40 % w/v of immobilized lipase loading and 0.2 M of substrate concentration at 38 °C during 24 h. The predicted yield was 92.78 %, and the yield obtained during synthesis was 93.48 %. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink