Comparison Between Xanthomonas campestris pv. manihotis (ISPP List 1980) and X. campestris pv. cassavae (ISPP List 1980) by Means of Phenotypic, Protein Electrophoretic, DNA Hybridization and Phytopathological Techniques
| Autor: | L. Meiresonne, J. De Ley, M. Van Den Mooter, Monique Gillis, Karel Kersters, J. Swings, Henri Maraite |
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| Rok vydání: | 1987 |
| Předmět: | |
| Zdroj: | Microbiology. 133:57-71 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/00221287-133-1-57 |
| Popis: | SUMMARY: Xanthomonas campestris pv. manihotis (ISPP List 1980) and X. campeststris pv. cassavae (ISPP List 1980) strains, isolated from cassava (Manihot esculenta) plants of different geographical origin, were studied by numerical analysis of 267 phenotypic features, computer-assisted comparison of gel electrophoregrams of soluble proteins, mol% G + C determinations, DNA: DNA hybridizations and virulence tests. X. campestris pv. manihotis and pv. cassavae constituted separate biological entities which could be differentiated from each other by four biochemical features, their symptoms on cassava, their soluble protein electrophoregrams and their DNA characteristics. Within each pathovar no correlation was found between phytopathogenicity, geographic origin and year of isolation of the strains, on the one hand, and the biochemical, physiological and protein electrophoretic properties on the other. Two yellowish Xanthomonas strains, CIAT 1164 and CIAT 1165, isolated from cassava in Colombia were genetically and electrophoretically similar to X. campestris pv. poinsettiicola, but were unable to infect Euphorbia pulcherrima. X. campestris pv. poinsettiicola was genetically more related to the X. campestris pv. manihotis cluster than to the X. campestris pv. cassavae cluster. |
| Databáze: | OpenAIRE |
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