CAR+ T cell anti-tumor efficacy revealed by multi-dimensional single-cell profiling
| Autor: | Xingyue An, Gabrielle Romain, Melisa Martinez-Paniagua, Irfan N Bandey, Jay R T Adolacion, Mohsen Fathi, Ivan Liadi, Fatemeh Sadeghi, Ankit Mahendra, Amit Amritkar, Badrinath Roysam, Harjeet Singh, Laurence J Cooper, Navin Varadarajan |
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| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 202:134.2-134.2 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | T cells engineered to express chimeric antigen receptor (CAR) targeting CD19 have shown promising clinical responses in patients with certain hematologic malignancies, however, it is desirable to be able to enrich cells with enhanced anti-tumor efficacy prior to infusion. We utilized a suite of high-throughput technologies with single-cell resolution, including Timelapse Imaging Microscopy In Nanowell Grids (TIMING) that integrates cytokine profiling to reveal that persistent motility of CD19- specific CAR T cells is correlated to desirable polyfunctionality (elimination of tumor cells and cytokine secretion), contributing to anti-tumor effects. We implemented a marker-free Boyden chamber-based method to enrich CAR+ T cells with persistent motility (motile cell). Integration of transcriptomic profiling, immune phenotyping and metabolism demonstrated that motile cells are more naïve-like with higher oxidative metabolism and spare respiratory capacity. Our result also revealed that the master metabolic regulator AMP kinase (AMPK) is required for CAR+ T cells with high motility. We used a xenograft leukemia mouse model (CD19+ NALM-6) and validated that the motile cells have enhanced persistence and superior anti-cancer effect in vivo compared to the parental un-sorted population. Collectively, our multi-dimensional results demonstrated that persistent motility is a selectable biomarker of expanded CAR+ T cell bioactivity. |
| Databáze: | OpenAIRE |
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