| Popis: |
High protein diet reduces food intake and improves glucose homeostasis in rodents and humans with obesity and diabetes, but the mechanistic site (s) of action remain unclear. Small intestinal lipid and glucose sensing regulates energy and glucose homeostasis, but the metabolic role of gut protein sensing remains elusive. In this study, we infused 8% casein hydrolysate as protein source into the upper small intestine (USI) and performed fasting-refeeding studies. We found that USI casein infusion (1.28 kcal/min) suppressed food intake up to 6h in chow but not high fat (HF) - fed rats (chow USI: sal 47±2 vs. cas 36±2 kcal, n=18, p In summary, these data illustrate that USI protein sensing increases glucose tolerance in chow and HF rats via CaSR, and lowers feeding in chow but not HF rats. Future studies are needed to dissect the metabolic role of CaSR not only for USI but also ileal protein sensing in energy balance and glucose homeostasis in obesity and diabetes. Disclosure R.J.W.Li: None. D.Barros: None. Y.Lim: None. S.Zhang: None. A.Gao: None. T.K.Lam: None. |
