Modified chitosan as a spacer for protein immobilization

Autor:	Meilutė Meizeraitytė, Gervydas Dienys, Rūta Gruškienė, Ricardas Makuska
Rok vydání:	2013
Předmět:	chemistry.chemical_classification Immobilized enzyme Inulin Proteolytic enzymes Biochemistry Catalysis Chitosan chemistry.chemical_compound Enzyme Dextran chemistry Side chain Organic chemistry Ethylene glycol Biotechnology
Zdroj:	Biocatalysis and Biotransformation. 31:181-189
ISSN:	1029-2446 1024-2422
DOI:	10.3109/10242422.2013.814645
Popis:	Three new, water-soluble, N-modified chitosan derivatives containing poly(ethylene glycol), dextran or inulin side chains were used as spacers for enzyme immobilization on a natural silk carrier. Amylolytic enzymes Maltogenase L and Promozyme D2, lipolytic enzyme Resinase HT and a complex of proteolytic enzymes from Streptomyces flavus 197 were immobilized. The activity of the immobilized enzymes and their stability during storage were similar to that obtained with synthetic polyamine—poly(ethylene imine) as a spacer. High operational stability of co-immobilized amylolytic enzymes Maltogenase L and Promozyme D2 in a continuous flow mini-reactor was demonstrated.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::ae140119a73875ea168035923bcdf421 https://doi.org/10.3109/10242422.2013.814645 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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