Comparative Analysis of Transgenic Tobacco Plants with Different Heterologic Plant Defensive Genes

Autor: Ya. E. Dunaevsky, E. Yu. Yakovleva, T. I. Odintsova, A. M. Kudryavtsev, K. V. Sydoruk, Tatyana V. Korostyleva, Mikhail A. Belozersky, E. A. Istomina, V. G. Bogush, N. V. Khadeeva
Rok vydání: 2020
Předmět:
Zdroj: Russian Journal of Genetics. 56:307-316
ISSN: 1608-3369
1022-7954
DOI: 10.1134/s1022795420030084
Popis: In order to study the possibility of creating new plant forms resistant to phytopathogens, a collection of transgenic plants of model tobacco culture with new different plant protective genes was obtained by the agrobacterial transformation method. First an addition of a collection with serine proteinase inhibitor BWI-1a (ISP) from buckwheat with fragments of a spidroin gene as putable enhancer by vector constructions different designs was done. Secondly, transgenic plants with an antimicrobial peptide from sinthetic wheat Triticum kiharae and with defensine from Stellaria media. Comparative study of physiological characteristics of transgenic plants in biotests in vivo (with isolated leaves) and in vitro (with well biotests) was carried out. Regardless of the design of the vector construction, the target genes were expressed to a different extent in the tissues of all transgenic plants and their seed and vegetative progenies and gave their tissues antibacterial activity, indicating the synthesis of the functional protein. The introduction to the tobacco tissues of the heterologic plant protective genes of different nature that plants use in different defense mechanisms led to a similar increase in antibacterial activity of the transgenic tobacco tissues.
Databáze: OpenAIRE
Externí odkaz: