Precipitation of VEGF from mesenchymal stem cell culture supernatant using the PCA process
| Autor: | Si-na Kim, Tae Jun Yoon, Youn-Woo Lee, Chan-Ju Lee, Sun U. Song, Won-Su Son, Hee Jeong Park |
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| Rok vydání: | 2019 |
| Předmět: |
Chromatography
Chemistry Precipitation (chemistry) General Chemical Engineering Mesenchymal stem cell 02 engineering and technology 021001 nanoscience & nanotechnology Condensed Matter Physics 030226 pharmacology & pharmacy Miscibility Supercritical fluid Vascular endothelial growth factor 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Scientific method Target protein Physical and Theoretical Chemistry 0210 nano-technology Mass fraction |
| Zdroj: | The Journal of Supercritical Fluids. 151:40-48 |
| ISSN: | 0896-8446 |
| DOI: | 10.1016/j.supflu.2019.05.008 |
| Popis: | Mesenchymal stem cell (MSC) culture supernatant solutions were dried under sub- and supercritical conditions using the precipitation with compressed antisolvent (PCA) process. Of the supernatant components, vascular endothelial growth factor (VEGF) was selected as the target protein. N-Methyl-2-pyrrolidone (NMP) was used as a co-solvent to enhance the miscibility of the supernatant with the antisolvent, CO2. The effects of temperature, pressure, NMP/solution mass fraction, and washing time were observed, and the conditions that allowed more efficient mixing among the solvents yielded the most amount of VEGF with retained activity. The products obtained by the optimum condition in this study were compared to a lyophilized sample. PCA processed samples produced more uniform and less agglomerated particles while exhibiting higher stability when reconstituted in water for 14 days. Although the PCA process was optimized for only VEGF, structures of additional 39 growth factors were also maintained in the dried samples. |
| Databáze: | OpenAIRE |
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