Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp. SD116
| Autor: | Zhijie Sun, Xiaojin Song, Ya-Jun Liu, Qiu Cui, Yu-rong Cheng, Yingang Feng, Zengxin Ma, Gu-Zhen Cui |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Cell growth 030106 microbiology food and beverages Fatty acid Dehydrogenase 03 medical and health sciences chemistry.chemical_compound Enzyme chemistry Biochemistry Docosahexaenoic acid Glucose-6-phosphate dehydrogenase lipids (amino acids peptides and proteins) sense organs NADPH regeneration Agronomy and Crop Science Polyunsaturated fatty acid |
| Zdroj: | Algal Research. 19:138-145 |
| ISSN: | 2211-9264 |
| DOI: | 10.1016/j.algal.2016.08.005 |
| Popis: | Aurantiochytrium sp. (Thraustochytriaceae, Stramenopiles) is a highly promising polyunsaturated fatty acid (PUFA) resource due to its high docosahexaenoic acid (DHA) production. The reducing agent NADPH is essential for the production of PUFA and other fatty acids, and glucose-6-phosphate dehydrogenase (G6PDH) is one of the most important enzymes for NADPH regeneration in Aurantiochytrium. To investigate whether PUFA production could be enhanced by stimulating G6PDH, a recombinant strain, termed SD116::G6PDH, was constructed by overexpressing G6PDH in Aurantiochytriumsp. SD116. Although decreased cell growth and fatty acid production were observed, G6PDH overexpression changed the fatty acid profile and enhanced the proportion of PUFA in lipids by 10.6%. These results confirmed the positive effect of G6PDH activity on PUFA production and suggested that the manipulation of NADPH production is a promising strategy for increasing PUFA production in Aurantiochytrium. (C) 2016 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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