On-Line Flow-Injection Chemiluminescence Determination of Bovine Serum Albumin Using Surfactant-Enhanced Dichlorofluorescein-Hypochlorite System

Autor:	Shengfu Wang, Kai Zhang, Wen-Yue Yang, Chun-Bao Huang
Rok vydání:	2010
Předmět:	Detection limit Flow injection analysis Chromatography biology Biochemistry (medical) Clinical Biochemistry Serum albumin Hypochlorite Biochemistry Analytical Chemistry law.invention chemistry.chemical_compound chemistry Dichlorofluorescein law Sodium hypochlorite Electrochemistry biology.protein Bovine serum albumin Spectroscopy Chemiluminescence
Zdroj:	Analytical Letters. 43:2275-2282
ISSN:	1532-236X 0003-2719
DOI:	10.1080/00032711003717331
Popis:	A flow injection technique combined with a chemiluminescence method was established for the determination of bovine serum albumin (BSA). Strong chemiluminescence was observed when BSA-dichlorofluoresce (DCF) complex was oxidized by sodium hypochlorite (NaClO) in an alkaline medium and in the presence of cetyltrimethylammonium bromide (CTAB). The reaction conditions of the chemiluminescence were carefully optimized. Under the optimal conditions, the method had a linear range of 0.01–20.0 μg/mL, with a detection limit of 0.007 μg/mL for BSA (3σ). The relative standard deviation of 1.0 μg/mL BSA (n = 8) is 1.4%. The method was applied to determine BSA in milk samples and it worked well.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::adf58a5555a6dd4dd449c5d02dc0b955 https://doi.org/10.1080/00032711003717331 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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